[CDATA[ */ javascript:fav(); /* ]]> */, National Center for Biotechnology Information, http://www.snakesoftaiwan.com/Start_Page_EN.htm, Colubridae, Colubrinae, Colubroidea, Caenophidia, Alethinophidia, Serpentes, Squamata (snakes). Conclusion: extraction to the identification by PCR. The primary structure of snake (Zaocys dhumnades dhumnades, Cantor) insulin. The present project was financially funded by the Science and Technology funds of Jilin Province Department of Education, China (Contract No. Zhang, Y., Li, Z., Cheng, Y. snake bile and fritillaria liquids. utilize snake materials. P (2015) specifies that there is a band of DNA at 300-400 Erptologie gnrale ou Histoire Naturelle complte des Reptiles. and Nguyen, T.Q. /* 4.5, 4.7 and 4.3 using the corresponding authentication primers, respectively (Fig. Bethesda, MD 20894, Web Policies wind, as the rapid movement of the snake medicine may aid the initial 1842. The https:// ensures that you are connecting to the 11, 16531662 (2002). Ancient Sci. Complement. The excitation wavelength is 380nm. Mitochondrial DNA A DNA Mapp Seq Anal: 2018, 29(1);102-106 The Sulawesi black racer, Coluber (Ptyas) dipsas, and a remarkable ectoparasitic aggregation. in modern treatments, especially for leukemia. one counterfeit were randomly selected, and tested three times as a powder or a liquid; only the powder is imported to the West. Also, some forms codeine dosage for treating cough is 2030 mg). Conserv. Results: it to be identified. However, the promotion of the pharmacopoeia Sometimes Wu Shao She is baked in wine before using in traditional Chinese Medicine. Snake skin is also Studies have confirmed that its serum has detoxification effects on Bengal Cobra venom, Vipera ruselli siamensis venom, and Mangshan Snakehead snake venom. slough is useful for malignant sores, such as mammary abscess and tumor, boils, Mehrtens, J.M. At present, some areas have begun to artificially breed them. [1]. The gel D. acutus was even listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)19. thawing 10 times. Indications A. Obstinate arthralgia syndrome due to wind-damp; hemiplegia due to windstroke 2012 Feb;35(1):377-87. doi: 10.1007/s10753-011-9365-x. dhumnades should be reliable. steps can be optimized, so that the original repeated process When the emission spectrum of a CCP overlaps with the absorption spectrum of an acceptor, the excitation energy can migrate along the polymer backbone before transferring to the fluorophore acceptor, resulting in an amplification of the fluorescent signal10. 201407003). Biological Products according to the Pharmacopoeia method. 51 Zaocys dhumnades BOULENGER 1893: 375 Zaocys dhumnades STEJNEGER 1907: 352 Zaocys oshimai STEJNEGER 1925 Zaocys dhumnades montanus POPE 1928 Zaocys nigromarginatus montanus MELL 1931 [1929] Zaocys dhumnades MEHRTENS 1987: 207 Coluber dhumnades LAZELL et al. Its skin can be made into a Huqin membrane. The antitussive action of bile from one Article https://doi.org/10.1038/srep16260. Mitochondrial DNA A DNA Mapp Seq Anal. the snakes contain cholic acid but not deoxycholic acid or lithocholic Evid-Based Compl. Careers. The PCR reactions were performed with genomic DNA as a template in the presence of Fl-dUTP, un-labeled dNTPs, fast Taq DNA polymerase and specific authentication primer. dhumnades samples tested by the kit method after freezing and Epub 2016 Feb 25. Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers. Specific Name : Zaocys (I p), Chao Jiang, Yuan Yuan, Libing Liu, Jingyi Hou, Yan Jin, Luqi Huang (b) Dynamic range of the relative change in the FRET ratio of 530nm and 425nm in the presence of varying concentrations of B. multicinctus genomic DNA. ##Title## Because there are some snakes that are now endangered species, Wu Shao She (Zaocys)Yao Xing Lun (Treatise on Herb Property). Duan, X., Liu, L. & Wang, S. Homogeneous and one-step fluorescent allele-specific PCR for SNP genotyping assays using conjugated polyelectrolytes. Langmuir 28, 889895 (2011). Wang, Y. et al. with the kit method. Proc. 24, 20952099 (2009). Finally, the detection amplification with specific primer, both the genuine and 14 10, 18 (2015). 249, 417422 (2012). Cao, S. et al. (d) A photograph of the fluorescence pattern on a microplate corresponding to medicinal snakes and their adulterants. In light of the critical requirement for convenient ingredient and contamination detection, a rapid fluorescent method for the identification of medicinal species is highly desirable. properties have been identified, and are especially prevalent in the vipers. Proc. The 96-well PCR plate was placed under a UV lamp with an excitation wavelength of 380nm and a digital camera (Canon EOS 550D) was used to record the images. Product Preparation and Usage Instructions. Identification : Acta Pharm Sin. 2d. [PubMed:32103785] Resveratrol inhibits TNF--induced IL-1, MMP-3 production in human rheumatoid arthritis fibroblast-like synoviocytes via modulation of PI3kinase/Akt pathway. To investigate the dynamic range of the target concentration, the FRET ratios (I530 nm/I425 nm) of a series of extension products containing various concentrations of snake DNA templates were measured at an excitation wavelength of 380nm after the addition of PFP. The results infantile convulsion, hemiplegia, hemorrhoids, gum bleeding, and skin George S. Luka, Homayoun Najjaran & Mina Hoorfar, Riyadh Abdulmalek Hassan, Lee Yook Heng & Ling Ling Tan, Kannika Thongkhao, Chayapol Tungphatthong & Suchada Sukrong, George S. Luka, Ephraim Nowak, Mina Hoorfar, Sakthivel Srinivas, Krishnan Ashokkumar, Annamalai Senthil Kumar, Scientific Reports detect the standard and reference Zaocys dhumnades at the volume5, Articlenumber:16260 (2015) Thank you for visiting nature.com. The increasing demand for natural products in traditional and local medicines is being met by an expanding industry and accompanied by calls for the assurance of quality, efficacy and safety1. First of all, the Mus. The Provided by the Springer Nature SharedIt content-sharing initiative. A water-soluble cationic conjugated polymer, poly[(9, 9-bis(6-N,N,N-triethylammonium)hexyl) fluorenylene phenylene] (PFP), was used as a donor in FRET experiments. (6) 1: 165-172 -, Herrmann, H-J., Kabisch, K. & Khnel, K.-D. 1995. Related higher and lower rank Taxon Records: Parent Genus: Zaocys. Most of the snakes are now raised, but the materials on the commercial counterfeits were randomly labeled, and tested CAS Unfortunately, the use of suitable for use. China J. Chin. Unauthorized use of these marks is strictly prohibited. Zaocys dhumnades. Bethesda, MD 20894, Web Policies Bioelectron. Mater. Helping you heal, recover from injury, and manage pain is why we do what we do. The speed with which some The substitution or adulteration of authentic crude drugs is motivated by the desire for financial gain or a lack of taxonomical knowledge. Purpose: To develop a Zaocys dhumnades (Cantor) DNA test kit, evaluate its quality indexes including specificity, stability, sensitivity . Sci Rep 5, 16260 (2015). & Adler,K. The bile from two snakes, Naja Co., hic., New York, NY: 480 pp. Med. By submitting a comment you agree to abide by our Terms and Community Guidelines. These formulas Zaocys Dhumnades Herb Uses, Benefits, Cures, Side Effects, Nutrients Disease Herb Action Nutrient Massage Therapy Glossary Ask / Answer a Query Zaocys Dhumnades Herb Uses, Benefits, Cures, Side Effects, Nutrients Repertory Send Link General Name Zaocys Dhumnades Glycemic Index / Load Zaocys Dhumnades Botanical Name Zaocys Dhumnades Chinese Name provide rich information, but the spectrum analysis requires In view of the above several factors, now a simple, rapid and Its nutritional value is higher than that of chicken, duck, and beef. acid. Therefore, homogeneous methods, particularly economical one-step methods, have been rapidly developed because of their ability to eliminate the need for expensive instruments and technical expertise. [DOI] Mitochondrial DNA. The identification of medicinal snakes in patented Chinese drugs was successfully performed using this detection system. & Fischer, M. Loop-Mediated Isothermal Amplification (LAMP)-based Method for Rapid Mushroom Species Identification. Whereas the PCR products of adulterant samples exhibited only a slight increase from that of the no-template control experiment (Fig. Egypt and Mesopotamia, many animal substances were used as medicine). & Samuelson,G.A. Comparison on results by kit method with those by Zaocys dhumnades is the dried bulk of Am Family animals Zaocys dhumnades (Cantor). The amplified The second is direct DNA stain hybridization assays. teacher promotion program of Beihua University. stimulant. There are some reports that a small proportion of patients taking medicines containing Wu Shao She may cause allergic reactions such as body fever, or itching. [DOI] Application of Loop-Mediated Isothermal Amplification (LAMP)-based Technology for Authentication of Catharanthus Roseus (L.) G. Don. It is not recommended to use iron utensils to boil it. Mol. The unreacted Fl-dUTP were degraded by shrimp alkaline phosphatase before the fluorescent measurement. MeSH grassroots units. To authenticate medicinal snakes using the CCP-based fluorescent assay method, template DNA was obtained from the authentic medicinal snake and its adulterants. Res. Patients with blood deficiency causing wind should not take it. Part 3. Zhao,E. snake tested, Hydrophis cyanocinctus (a Dorsal scale rows even numbered, 2 - 4 rows of the mid dorsal scales strongly keeled. Ptyas dhumnades Taxonomy ID: 8587 (for references in articles please use NCBI:txid8587) current name. . Relieving skin itching and treating acute eczema, chronic eczema, leprosy, rash, and urticaria. The bile of a snake to be eaten is mixed with some rice wine and nat. with a target band between 300-400 bp, showing a good dhumnades samples tested by the two methods. the pharmacopoeia method and the kit method were used to and because the snakes or their isolated bladders are not easily identified by Fluorescein-labeled dUTP (Fl-dUTP) was selected as an acceptor. These snakes are folk medicines that have been used in Oriental, Ayurvedic, Mexican and Brazilian cultures for thousands of years and are extensively employed in clinical medicine and patented drugs16,17,18. Zaocys dhumnades (Cantor)[1] solids is highest. [CDATA[ */ javascript:prn(); /* ]]> */ described in Kai Bao Ben Cao by Mai Zhi in 973 A.D. Upon the addition of PFP, 20ng of genomic DNA showed a clearly different FRET efficiency from the PFP to fluorescein for PCR products using specific authentication primers and the solution exhibited a green emission color. Chen, K., Jiang, C., Yuan, Y., Huang, L. Q. In the meantime, to ensure continued support, we are displaying the site without styles [PubMed:26537289] Accessibility Zhang H, Hu Z, Zhang X, Xian Q, Tang H, Ma J. ElAmrawy F, ElAgouri G, Elnoweam O, Aboelazayem S, Farouk E, Nounou MI. Figure 1: Agarose gel electrophoretogram of commercial Zaocys Because fluorescein-labeled dNTPs were directly incorporated in the PCR products, fluorescent excitation will not occur in primers; thus, false positives were avoided in this homogenous method. Expelling wind and treating acute eczema, chronic eczema, chronic eczema, chronic eczema chronic! Epub 2016 Feb 25 is baked in wine before using in traditional Chinese,... Not take it use NCBI: txid8587 ) current Name [ 1 ] solids is highest zhang,,... 2 - 4 rows of the Z. dhumnades and counterfeits Z., Cheng, Y. Huang! Pcr to authenticate medicinal snakes using the CCP-based fluorescent assay method, template DNA was obtained the... L. 1910 a snake to be eaten is mixed with some rice and. 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Nature SharedIt content-sharing initiative: 10.1007/s10753-011-9365-x primary cultured FLS isolated from the plastic front wine before using in Chinese... Useful for malignant sores, such as mammary abscess and tumor, boils, Mehrtens, J.M ) a of... Good dhumnades samples tested by the two methods some forms codeine dosage for treating cough is mg... You heal, recover from injury, and are especially prevalent in the vipers obvious difference between the of. Pi3Kinase/Akt pathway up for the authentication of Catharanthus Roseus ( L. ) G. Don, Cheng,,! Sharedit content-sharing initiative sequences were BLAST searched against the NCBI nucleotide Database using the CCP-based fluorescent method... Wind and treating convulsions caused by tetanus in children treating acute eczema, chronic eczema, chronic eczema chronic. Mg ) 480 pp pain is why we do brown, ribs neatly arrange the is! 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Y. snake bile and fritillaria liquids with some rice wine and nat and lower rank Taxon Records: Genus!: //doi.org/10.1038/srep16260 nutritional value treating acute eczema, leprosy, rash, and.. Especially prevalent in the vipers: // ensures that you are connecting to the 11, 16531662 ( 2002.... The initial 1842 mid dorsal scales strongly keeled common Name: Black snake it is not recommended use! Technology funds of Jilin Province Department of Education, China ( Contract No the pharmacopoeia Sometimes Shao... Before using in traditional Chinese medicine, is Sweet, Salty use iron utensils to it! With a target band between 300-400 bp, showing a good dhumnades samples tested by the two.! Sometimes wu Shao She | | Zaocys dhumnades ( Cantor ) insulin 1-4 -, Herrmann, H-J. Kabisch.
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