These tools for evaluating information may be useful: Categories: National Library of Medicine Lund University Intracellular reverse transcription of Pfizer BioNTech COVID-19 mRNA vaccine BNT162b2 in vitro in human liver. Every cell within our human body is undergoing regular checks, and when you get an abnormal cell, that abnormal cell is then broken down and deleted, Strain said. This three-part series was aimed at describing several important aspects of science. Where that paper was getting a lot of press was the idea that those pieces of DNA were then getting incorporated into the genome, and theres absolutely no evidence that that happened, Moore said. By using our site, you acknowledge that you have read and understand our Privacy Policy Because the study design does not necessarily reflect what happens in most human bodies, he said the findings cannot be extrapolated to make inferences about what would happen in human subjects. PCR on genomic DNA of Huh7 cells exposed to BNT162b2 amplified the DNA sequence unique to BNT162b2. (In-vitro Swedish Study) https://youtube.com/live/MjxlvduyJyc?feature=share 28 Feb 2023 02:39:58 Its precisely what health experts and fact-checkers said for more than a year could not occur. Neither your address nor the recipient's address will be used for any other purpose. This is 1 replication competent plasmid per 350 mRNA molecules and equates to billions of antibiotic resistant plasmids injected per person per shot . some scientists have suggested that integration of SARS-CoV-2 genetic material into human cells could be why some people still test positive by PCR well after they have recovered from their infection. A cancer vaccine seemed a far-fetched idea just a few years ago, but now the shots exist and are gaining steam in the biotech community. The authors declare no conflict of interest. Bad Bunny. YDM: One of the limitations of our study is that we dont know if what we observed in this cell line could also happen in cells of other tissue types, and this needs to be addressed in follow-up studies. -, Polack F.P., Thomas S.J., Kitchin N., Absalon J., Gurtman A., Lockhart S., Perez J.L., Perez Marc G., Moreira E.D., Zerbini C., et al. Indeed, about 8 percent of human DNA comes from viruses inserted into our genomes during evolution. Antibody levels were also measured in separate tests. A gene that the researchers studied produces the protein LINE-1. Importantly, LINE-1 has been associated with various disease-related conditions, including cancer. While the Moderna vaccines are meeting this specification, the Pfizer vaccines are 10 fold higher in contamination with 1 DNA molecule per 350 mRNAs. They certainly tell us something we did not know without the study, but it is important to understand the limitations of the data. Coronaviruses, however, are not expected to do this, said Bethany Moore, chair of the University of Michigans Department of Microbiology and Immunology. The author described two potential mechanisms by which this could occur. PCR primer set used to detect mRNA level and reverse-transcription of BNT162b2. Available for free at the Rakuten Viber Sticker Market from November 18th! 5. In a study published in Cell Reports, researchers from Lund, Patients are often asked to rate their feelings using a rating scale, when talking to psychologists or doctors about their mental health. WebPrevious vaccines were largely attenuated or live vaccines that were bioengineered to not cause as severe of a reaction while still being similar enough to the origin virus to cause us to gain immunity. Pfizer did not comment on the studys findings, the Epoch Times reported, stating only that its mRNA vaccine does not alter the human genome. Dr. Peter McCullough, an internist, cardiologist, and epidemiologist,wrote on Twitterthat the Swedish studys findings have enormous implications of permanent chromosomal change and long-term constitutive spike synthesis driving the pathogenesis of a whole new genre of chronic disease.. Whether our results are true for other cell types in humans, or if they are specific to mRNA vaccines, are among many questions for further research. WebIf proto-oncogenes or tumor suppressor genes mutate, cells can become cancerous. selenagomez. The mRNA vaccines are entirely synthetic using lab synthesized RNA and lipid nanoparticles for delivery. By Meiling Lee. In the case of Covid shots, the vaccines from Moderna and Pfizer ( PFE )/BioNTech introduce a single spike protein. The Swedish scientists have found that the messenger RNA or mRNA found in the Pfizer COVID vaccine becomes DNA in liver cells. The views expressed in this article are those of the author and do not reflect the official position of yourNEWS. doi: 10.1128/Spectrum.01162-21. Reuters has previously fact checked the claim that mRNA vaccines alter human DNA (here), (here), (here) and (here). In a lab environment, i.e. Studies comparing the amount of live virus shed during infection with the amount of RNA detected by PCR have not shown the two measures to be well correlated. What are the next steps in this research? Our publication is the first in vitro study on the conversion of mRNA vaccine into DNA, inside cells of human origin. Unlike the abnormal cells used in the study, the human bodys protections would help stop imported genetic material from being corrupted, Strain said. So, one may quickly realize that with 20 building blocks and tens or hundreds of thousands of proteins, small (sometimes even large) stretches of overlap are likely to occur. WebPfizer Vaccine Becomes DNA in Liver Cells. Bethesda, MD 20894, Web Policies Rasmussen also said, cell lines differ from cells in living organisms, and therefore it is important that similar investigations are also studied in humans.. And, while this may be the case early during infection, there are a few additional considerations when thinking about these data: Hopefully these examples demonstrated some of the ways that a paper should be examined. Illustration of BNT162b2 was adapted from previously described literature [34]. The whole process of Pfizers mRNA transfecting liver cells occurred within just six hours, per the study. CLAIM: A Swedish study shows that Pfizers COVID-19 vaccine changes recipients DNA. Pathogenic priming likely contributes to serious and critical illness and mortality in COVID-19 via autoimmunity. Lund Universitymagnus [dot] rasmussen [at] med [dot] lu [dot] se, Yang de Marinis The mRNA in the COVID-19 vaccines helps train the body to recognize a protein from the coronavirus to trigger an immune response. As such, finding similar stretches of genes is not novel, unlikely, or particularly surprising. The authors said more investigations are needed, including in whole living organisms such as animals, to better understand the potential effects of the mRNA vaccine. COVID-19 Vaccines (Pfizer-BioNTech and Moderna) How They Work; Happy New Year 2021! Your email address is used only to let the recipient know who sent the email. The CDC says that the COVID-19 vaccines do not change or interact with your DNA in any way, claiming that all of the ingredients in both mRNA and viral vector COVID-19 vaccines (administered in the United States) are discarded from the body once antibodies are produced. Indeed, if you look at the article in the journal in which it was published. An important thing to notice when looking at this figure is that unlike most axis numbers, those on the y-axis showing cycle threshold are decreasing in number as they go higher on the graph. MR: We understood that the study would attract attention, but we think it is self-evident that this type of research should be pursued. PCR was run on all samples with primers targeting BNT162b2, as shown in Figure 1 and Table 1. However, this is not an appropriate conclusion from this paper for several reasons: Logical fallacy called hasty generalization (It is important to note in this case, that the misconception was not because of the quality of the science or the messaging of the authors, but rather because others took the findings out of context.). RNA is closely related to DNA, and one type, called messenger RNA or mRNA, sends instructions to the cell for different purposes. The results have in many cases been misinterpreted, the Q&A also stated. WebThis and similar observations caused Spanish scientists to become curious about that actual contents of the vaccinations, since they wanted to understand what was giving rise to the magnetic effects. Your browser has javascript turned off or blocked. doi: 10.1056/NEJMc2107717. And, notably, there didnt appear to be a control group for the hep B vaccine Siri says. Two different antibody-dependent enhancement (ADE) risks for SARS-CoV-2 antibodies. Contact us
It is known from animal studies that it is also expressed early in embryonic development", explains Yang de Marinis. Further proof with COVID-19. In part II, we saw that sometimes big discoveries do come along, but they dont magically change our thinking overnight. Once all samples were plotted, an average across samples was added by insertion of a line plot. "LINE-1 can convert RNA to DNA and has been shown to be found in tissues, including stem cells, in the human body. 3401 Civic Center Blvd. Our COVID-19 vaccine does not alter the DNA sequence of a human cell, a Pfizer spokesperson told The Epoch Times in an email. YDM: A previous study from MIT has indicated that the SARS-CoV-2 virus mRNA can be converted to DNA and integrated into the human genome. The Centers for Disease Control and Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line. In the general population, the antibody response wanes over the course of time after two doses of the vaccination, but seems to be more Once the string of amino acids is created in the cell, chemical and physical forces, such as the charges and shapes of the amino acids in the sequence as well as other factors in the cellular environment, cause the newly formed protein to fold into a 3D shape. AP'S ASSESSMENT: False. Link to the publication in Molecular Biology: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line. WebThis leads to immune responses that ultimately destroy those cells. WebPosts on social media have claimed that the mRNA COVID vaccines such as the Pfizer vaccine and Moderna vaccine could alter a persons DNA. The information you enter will appear in your e-mail message and is not retained by Medical Xpress in any form. Study: Pfizer Covid Vaccine Converts to DNA Inside Liver Cells. Likewise, the Results section has five sentences. yourNEWS is a hyper-local social news and advertising platform. 2022 Apr;28(4):612.e1-612.e7. This in itself motivates the need for further studies. Pfizer didnt comment on the findings of the Swedish study and said only that its mRNA vaccine does not alter the human genome. 2021 Jun 7;22(5):172. doi: 10.1208/s12249-021-02058-y. 2021;385:759760. You should not use it to replace any relationship with a physician or other qualified healthcare professional. It takes time and a significant accumulation of evidence to confirm them. DETAILS - Pfizer Vaccine Becomes DNA in The Human Liver Cells Huh7. Detection of DNA amplicons of BNT162b2 in Huh7 cells treated with BNT162b2. Click here:\rhttps://www.drbeen.com/yt-special-p/\r\rWant to support this work? On 13th of February, a transplant of stem cell-derived nerve cells was administered to a person with Parkinsons at Skne University Hospital, Sweden. Materials in this section are updated as new information and vaccines become available. in petri dishes, the researchers added Pfizer BioNTech's vaccine to a cell line that originally came from a human liver tumour. (in-vitro swedish study) https://lnkd.in/df6hkugy Some genetic mutations are inherited in the DNA that parents pass on to their children at birth, including gene changes that are known to increase the risk of developing cancer. It is reverse transcribed by LINE-1 proteins of the cells. MR: One should consider that cell lines differ from cells in living organisms, and therefore it is important that similar investigations are also studied in humans. The results found that the vaccine intracellularly integrates into a persons DNA in a process that can happen within six hours of exposure. Table S1 summaries the vaccines If you take just one thing away from this series, we hope you will have come to realize that science does not easily fit into a sound bite and, most often, it is not an either-or proposition, so if something sounds too simple, you probably need to ask more questions. Markus Aldn et al, Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line, Current Issues in Molecular Biology (2022). Earlier this year, the UK government announced it was partnering with BioNTech, who contributed to the development of the Pfizer COVID-19 vaccine. Spetzofai is a rustic spicy Greek dish that is made with thick sausages and long green and red peppers in a rich tomato sauce. This genetic material contains instructions the body's cells use to build a protein found in a bacterium or virus, and that protein is then recognized by the immune system. YDM: The attention of the media and the general public reflects a concern among some regarding new vaccine technologies. Two of the authors, Associate Professor Yang de Marinis (YDM) and Professor Magnus Rasmussen (MR), share their views. hasty generalization and appeal to ignorance. Telephone: +46 (0)46 222 00 00 (switchboard)
This would be like saying that two people have the same social security number because both contain the number 5. eCollection 2022 Dec. Mavridis C, Aidonidis G, Evangelou M, Kalogeridis A. Hist Philos Life Sci. (In-vitro Swedish Study) An accepted and published Swedish study demonstrates for the Said another way, it was an . Parents PACK. As we discussed in, The quantity of a genetic tool called LINE-1, The presence of DNA sequences similar to the vaccine RNA in the nuclei of these cells, Perhaps most importantly, this experiment was done on cells being grown in a lab. MR: The study was performed on human liver cells from one cell line cell cultures used for research purposes. Our publication is the first in vitro study on the conversion of mRNA vaccine into DNA, inside cells of human origin. Phase I/II study of COVID-19 RNA vaccine BNT162b1 in adults. In the study, the authors infected a human liver cell line with the Pfizer version of the COVID-19 vaccine. Further studies are needed to demonstrate the effect of BNT162b2 on genomic integrity, including whole genome sequencing of cells exposed to BNT162b2, as well as tissues from human subjects who received BNT162b2 vaccination, the authors said. Suggested accounts. Please select the most appropriate category to facilitate processing of your request. The https:// ensures that you are connecting to the Each dot on the table represents a study subject. This site uses cookies to assist with navigation, analyse your use of our services, collect data for ads personalisation and provide content from third parties. Two people in their 60s who received the shot were diagnosed with Guillain-Barre syndrome, out of about 20,000 vaccine recipients who participated in Pfizers Phase 3 Errors in interpretation can come from biased authors, lack of understanding of the science or the scientific methodology, or even a decision to intentionally misrepresent the work. Click here:\rhttps://www.drbeen.com/yt-special-p/\r\rWant to support this work? This is currently how depression and, Small passerine birds, such as blue and great tits, avoid breeding in urban areas where there are many non-native trees. and Terms of Use. Invasion of Ukraine: Russia and Ukraine ready for second round of talks tonight. One, theyre RNA or mRNA vaccines, and two, they seem to be very effective, at least in the limited clinical trials theyve done. But keep in mind, when the mRNA or adenovirus particles do hit cells outside of the liver or the site of injection, they're still causing them to express Spike protein anchored on their surfaces, not dumping it into the circulation. Anti-vaccination and ivermectin proponents have gone bananas over the new study from the Lund University YDM: This study does not investigate whether the Pfizer vaccine alters our genome. They used different concentrations of the vaccine and multiple timepoints to measure: Some pointed to this paper as proof that COVID-19 vaccines alter DNA. MR: There is no reason for anyone to change their decision to take the vaccine based on this study. We have a new vaccine, and it needs to be tested in cell and animal models and also in humans, in various ways. We saw that there was DNA converted from the vaccine's mRNA in the host cells we studied. Unauthorized use of these marks is strictly prohibited. And even if there was, what are you going to do with 147 kids' data?7 It did not assess whether the vaccine alters the human genome, or what the effects of that would be, according to experts and the study authors. Clipboard, Search History, and several other advanced features are temporarily unavailable. There is a crucial difference between mRNA and DNA. The. Current Issues in Molecular Biology. Press Office
It was assumed that the vaccines spike protein would remain mostly at the injection site and last up to several weeks like other proteins produced in the body. This has been the question our study aims to answer. No information in this video should ever be considered as a substitute for advice from a healthcare professional.\r\rURL list from Friday, Feb. 25 2022\r\rCIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML\rhttps://www.mdpi.com/1467-3045/44/3/73/htm\r\rFrontiers | Identification of Characteristic Genomic Markers in Human Hepatoma HuH-7 and Huh7.5.1-8 Cell Lines | Genetics\rhttps://www.frontiersin.org/articles/10.3389/fgene.2020.546106/full#:~:text=HuH%2D7%20(hereafter%20Huh7),experimental%20substitute%20for%20primary%20hepatocytes. Researchers found that the entire process of the Pfizer COVID vaccine becoming DNA in liver cells took about six hours. Fax: +46 (0)46 222 47 20, Invoice address: Box 188, 221 00 Lund
J. Med. 19.8M. Original video "Pfizer Vaccine Becomes DNA in Liver Cells. The vaccines mRNA converting into DNA and being found inside the cells nucleus is something that the Centers for Disease Control and Prevention (CDC) said would not happen. This is even more necessary today as information is easily created and even more easily shared. They have somewhere between 90 to 95% efficacy at providing immunity to someone We saw that there was DNA converted from the vaccine's mRNA in the host cells we studied. Why liver cells and why the specific dose? The study has received a lot of media attention, what are your thoughts on that? YDM: More research is needed. The Food and Drug Administration has flagged Guillain-Barre syndrome, a rare neurological condition, as a potential risk for Pfizers respiratory syncytial virus vaccine. We show that the vaccine enters liver cells as early as 6 hours after the vaccine has been administered. doi: 10.7759/cureus.32361. These vaccines deliver genetic material that instructs cells to begin making spike proteins found on the surface of SARS-CoV-2 that causes COVID-19 to produce an immune response. As such, by definition, a modeling study cannot serve as proof. MR: The study was performed on human liver cells from one cell linecell cultures used for research purposes. And this could be seen with natural virus infection as well, Moore said. Nature. Huh7 cells, Detection of DNA amplicons of BNT162b2 in Huh7 cells treated with BNT162b2. As such, even a rather long stretch of shared amino acids is not going to make two proteins automatically recognizable by the same antibodies because one or the other of the similar portions may be hidden within the 3D shape. At this stage, we do not know if DNA reverse transcribed from BNT162b2 is integrated into the cell genome. Huh7, MeSH It is important to bear in mind that the liver cells in this study are more genetically unstable than our own liver cells. 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Enhancement ( ADE ) risks for SARS-CoV-2 antibodies media attention, what are your thoughts that... Genes is not novel, unlikely, or particularly surprising the host cells we.. Moderna ) How they work ; Happy new Year 2021 sent the email - Pfizer vaccine Becomes DNA liver. Of antibiotic resistant plasmids injected per person per shot physician or other qualified healthcare.! Sometimes big discoveries do come along, but they dont magically change our thinking overnight reverse! Thinking overnight human liver cells as early as 6 hours after the vaccine intracellularly integrates into persons. 0 ) 46 222 47 20, Invoice address: Box 188, 221 00 Lund J... Today as information is easily created and even more necessary today as information easily... To serious and critical illness and mortality in COVID-19 via autoimmunity to answer are unavailable! Are your thoughts on that dot on the conversion of mRNA vaccine into DNA, inside cells human! Moderna vaccine could alter a persons DNA in liver cells Huh7 any other purpose: 10.1208/s12249-021-02058-y social news advertising. 7 ; 22 ( 5 ):172. doi: 10.1208/s12249-021-02058-y to billions of antibiotic resistant plasmids per... Be used for any other purpose created and even more necessary today as information is easily created even. Of BNT162b2 in vitro in human liver cell line with the Pfizer version of the data nanoparticles for delivery there!